Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode
نویسندگان
چکیده
منابع مشابه
Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope
We describe a technique based on moment-analysis for the measurement of the average number of molecules and brightness in each pixel in fluorescence microscopy images. The average brightness of the particle is obtained from the ratio of the variance to the average intensity at each pixel. To obtain the average number of fluctuating particles, we divide the average intensity at one pixel by the ...
متن کاملFluorescence correlation spectroscopy, raster image correlation spectroscopy, and number and brightness on a commercial confocal laser scanning microscope with analog detectors (Nikon C1).
Fluorescence correlation spectroscopy (FCS) was developed in 1972 by Magde, Elson and Webb. Photon counting detectors and avalanche photodiodes have become standards in FCS to the point that there is a widespread belief that these detectors are essential to perform FCS experiments, despite the fact that FCS was developed using analog detectors. Spatial and temporal intensity fluctuation correla...
متن کاملA custom confocal and two-photon digital laser scanning microscope.
We describe a custom one-photon (confocal) and two-photon all-digital (photon counting) laser scanning microscope. The confocal component uses two avalanche photodiodes (APDs) as the fluorescence detector to achieve high sensitivity and to overcome the limited photon counting rate of a single APD ( approximately 5 MHz). The confocal component is approximately nine times more efficient than our ...
متن کاملdetermination of retardation effect of som on aqueous leaching of polycyclic aromatic hydrocarbons using confocal laser scanning microscope
influence of soil organic matter (som) on the adsorption, desorption and transport of polycyclic aromatic hydrocarbons (pahs) has already been given full investigation in soils. in this paper, confocal laser scanning microscope (clsm) was used to elucidate aqueous leaching of pahs in different types of soil with soil columns, which enhanced understanding on transport of pahs appreciably. four ...
متن کاملdetermination of retardation effect of som on aqueous leaching of polycyclic aromatic hydrocarbons using confocal laser scanning microscope
influence of soil organic matter (som) on the adsorption, desorption and transport of polycyclic aromatic hydrocarbons (pahs) has already been given full investigation in soils. in this paper, confocal laser scanning microscope (clsm) was used to elucidate aqueous leaching of pahs in different types of soil with soil columns, which enhanced understanding on transport of pahs appreciably. four t...
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ژورنال
عنوان ژورنال: Microscopy Research and Technique
سال: 2007
ISSN: 1059-910X,1097-0029
DOI: 10.1002/jemt.20526